Abstract: The diets and environments of fossil rhinocerotoids from the Linxia Basin, Gansu, China,ranging in age from 25 to 2.5 Ma, were reconstructed based on bulk and serial carbon (C) and oxygen (O) isotope analyses of tooth enamel. The results support many previous hypotheses inferred from dentition and cranial and limb morphology and offer new insight on the paleoecology of some genera. The isotopic results support the following previous hypotheses: the Late Oligocene rhinoParaceratherium inhabited a forested environment, and the coexisting rhino Allacerops lived in a relatively open habitat and had a less specialized diet; the Middle Miocene Hispanotherium grazed in open territory, whereas the contemporaneous Alicornops had a more generalized diet in a forested environment; and the Late Miocene rhino Parelasmotherium grazed in an open steppe habitat. The isotope data indicate that the rhinos Acerorhinus and Dicerorhinus inhabited open steppe environments, inconsistent with previous inferences that these two rhinos dwelled in forested environments. The isotopic results are not conclusive concerning the habitat of Iranotherium, but support previous hypotheses that this rhino was a specialized C3 grazer. The results also suggest thatChilotherium was a forest-dweller throughout much of the Late Miocene, but occupied a more open environment by the end of the Late Miocene. Additionally, the results are consistent with previous hypotheses that the Pliocene rhino Shansirhinus and the Pleistocene rhino Coelodonta were grazers in open habitats. Finally, the C isotope data support that all rhinos in this study were pure C3 feeders,confirming that C4 grasses were not an important component of the plant biomass in the Linxia Basin from 25 to 2.5 Ma.