Abstract: The incisor enamel microstructure of Mina hui Li et al., 2016 from the upper Wanghudun Formation, Middle Paleogene of Qianshan, Anhui is described following the hierarchical system ranging from crystallites to Schmelzmuster. The enamel sample was taken from the upper incisor of the holotype. The enamel consists of a very thin prismless external layer, a relatively thick outer layer (PE) with radial enamel and an inner layer (PI) with multilayered Hunter-Schreger bands (HSB). The thickness of HSB is irregular and the inclination has a small angle; no transition zones between the HSB; prism cross sections are irregularly round; interprismatic matrix is very thick with crystallites in the PE running parallel to the prism long axes. These characters resemble pauciserial HSB in rodents.Ribbon-like prism seams and convergent crystallite discontinuities areconsistent in different portions of the enamel, which are interpreted as a primitive feature for Glires, or even placental mammals. Compared with known enamel structure of basal Glires, the enamel of Minastands for the earliest known evidence of double-layered enamel of the group. It indicates that the early evolution of Glires and placental mammals is complex and adds more details to the enamel microstructure of the earliest Glires.